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phos-akt (ser473) polyclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phos-akt (ser473) polyclonal antibody
    Phos Akt (Ser473) Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phos-akt (ser473) polyclonal antibody/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    phos-akt (ser473) polyclonal antibody - by Bioz Stars, 2026-03
    90/100 stars

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    FIGURE 6 SP140 inhibitor suppress glioma progress <t>and</t> <t>TRIM22/PI3K/AKT</t> signaling pathway. (A) Coexpression analysis of TRIM22 and SP family in TCGA glioma database. (B) The mRNA and protein expression level of TRIM22 in different cell lines. (C) The mRNA and protein expression level of TRIM22 in U87 and U251 after GSK761 intervention. (D) CCK-8 analysis showed cell proliferation in U87 and U251 after GSK761 intervention. (E, F) Wound-healing assay showed cell migration in U87 and U251 after GSK761 intervention. (G, H) Transwell invasion assay showed cell invasion in U87 and U251 after GSK761 intervention. (I) The mRNA expression level of TRIM22 after GSK761 treatment. (J) Western Blot analysis showed PI3K/AKT signaling pathway changes after GSK761 intervention. *p < .05, **p < .01, ns indicates no significance.
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    FIGURE 6 SP140 inhibitor suppress glioma progress and TRIM22/PI3K/AKT signaling pathway. (A) Coexpression analysis of TRIM22 and SP family in TCGA glioma database. (B) The mRNA and protein expression level of TRIM22 in different cell lines. (C) The mRNA and protein expression level of TRIM22 in U87 and U251 after GSK761 intervention. (D) CCK-8 analysis showed cell proliferation in U87 and U251 after GSK761 intervention. (E, F) Wound-healing assay showed cell migration in U87 and U251 after GSK761 intervention. (G, H) Transwell invasion assay showed cell invasion in U87 and U251 after GSK761 intervention. (I) The mRNA expression level of TRIM22 after GSK761 treatment. (J) Western Blot analysis showed PI3K/AKT signaling pathway changes after GSK761 intervention. *p < .05, **p < .01, ns indicates no significance.

    Journal: Brain and behavior

    Article Title: SP140 inhibitor suppressing TRIM22 expression regulates glioma progress through PI3K/AKT signaling pathway.

    doi: 10.1002/brb3.3465

    Figure Lengend Snippet: FIGURE 6 SP140 inhibitor suppress glioma progress and TRIM22/PI3K/AKT signaling pathway. (A) Coexpression analysis of TRIM22 and SP family in TCGA glioma database. (B) The mRNA and protein expression level of TRIM22 in different cell lines. (C) The mRNA and protein expression level of TRIM22 in U87 and U251 after GSK761 intervention. (D) CCK-8 analysis showed cell proliferation in U87 and U251 after GSK761 intervention. (E, F) Wound-healing assay showed cell migration in U87 and U251 after GSK761 intervention. (G, H) Transwell invasion assay showed cell invasion in U87 and U251 after GSK761 intervention. (I) The mRNA expression level of TRIM22 after GSK761 treatment. (J) Western Blot analysis showed PI3K/AKT signaling pathway changes after GSK761 intervention. *p < .05, **p < .01, ns indicates no significance.

    Article Snippet: The primary antibodies used in this study were anti-PI3K antibody (67121-1-Ig, Proteintech), anti-AKT antibody (60203-2-Ig, Proteintech), anti-pho-AKT antibody (80455-1-RR, Proteintech), anti-TRIM22 antibody (ab68071, Abcam).

    Techniques: Expressing, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Western Blot